Hito CryoMyelinStain™ Kit - Free Sample - Restrictions Apply

Hito CryoMyelinStain™ Kit - Free Sample - Restrictions Apply

Hito Golgi-Cox OptimStain™ Kit - Free Sample - Restriction Apply

$0.00

Try It Before You Buy It 
For certain products, customers can order free samples for testing purpose before purchasing the standard products. Customers only need to pay for the shipping and handling fee. Free samples are only available for researchers in the universities, colleges or research institutions. Limit one free sample per customer or address. We reserve the right to limit the distribution of free samples. If you have any questions about the free samples or have problems ordering free samples online, please contact our Customer Service center.

Hito Golgi-Cox OptimStain™ Kit designed based on the methods described by Glaser and Van der Loos, this kit makes dramatic improvement of the Golgi-Cox technique. The procedures are simplified and the processing time is greatly reduced. This kit delivers stable and improved staining quality, with minimal overstains and artifacts when used properly. Standard Kit can be used for 50 mouse brains. Hito Golgi-Cox OptimStain™ Kit has been tested extensively on the brains, spinal cords and hearts from several species of animals and proven to be sensitive for demonstrating morphological details of neurons and gila, It is a simple solution for your research.

Out of stock
SKU
HTKNS1125-FR

Golgi-Cox staining, also known as Golgi-Cox impregnation, is one of the most elegant and effective procedures for studying the morphology of neurons and glia. It remains a primary technique for visualizing dendritic branching patterns and dendritic spines, allowing isolation and visualization of dendritic arbours from a small random fraction of neurons in a given brain area. Golgi techniques are widely used not only for anatomical studies but also in research examining behavioral-morphological relationships. Applications include identifying autonomic innervations in the heart and peripheral tissues.

Traditional Golgi-Cox staining is time-consuming, requires a vibratome, yields relatively few stained cells, and often produces inconsistent results. The Hito Golgi-Cox OptimStain™ Kit, designed based on methods by Glaser and Van der Loos, provides significant improvements. Procedures are simplified, processing time is reduced, and staining quality is stable and reliable with minimal overstaining and artifacts.

Users can employ a cryostat set at –19ºC for sectioning, or alternatively prepare paraffin sections with a microtome. The Standard Kit supports up to 50 mouse brains. Extensively tested on brains, spinal cords, and hearts from several animal species, this kit is sensitive for demonstrating morphological details of neurons and glia.

Kit Contents Standard Kit Small Kit Sample Kit
Solution-1A (Stock Solution) 100 ml 50 ml 10 ml
Solution-1B (Prepare Solution) 150 ml 75 ml
Solution-2 250 ml 125 ml 10 ml
Solution-3 500 ml 250 ml 20 ml
Solution-4 250 ml 125 ml 20 ml
Solution-5 250 ml 125 ml 20 ml
Dropper Bottle (30 ml Solution-3) 1 1 1
User Manual and MSDS 1 1 1

Required Equipment / Materials (not included):

  • Cryostat (capable of cutting 80–200 µm thick sections at –19ºC)
  • Dry ice, O.C.T. compound, isopentane, ethanol, xylene, double-distilled or deionized water
  • Plastic/glass tubes or vials
  • Gelatin-coated slides (recommended: Hito Dual-Safe Gelatin-coated Slide Cat# HTHS0102 ) and coverslips
  • Staining jars for slide washing
  • Resinous mounting medium
  • Light microscope

pdf Hito Golgi-Cox OptimStain™ Kit Manual and MSDS
photo Golgi-Cox high definition sample image

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