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Scott’s Tap Water Substitute is a gentle formulation of a “bluing” reagent designed for histology and cytology. It blues hematoxylin gently while minimizing the loss of tissue sections and cells from glass slides.
Our modified Scott’s Tap Water Substitute provides a gentle yet effective bluing solution for staining. This formulation ensures rapid bluing and delivers precise detail of nuclear chromatin and nuclear membranes when used with routine Hematoxylin and Eosin stains.
Supplied as a 5x concentrated solution, it can be diluted to prepare up to 1250 ml of working solution, offering convenience and cost efficiency for laboratory use.
H&E staining, or hematoxylin & eosin staining, is one of the most widely used methods in histology and remains the standard stain in medical diagnosis and research.
The Hito Hematoxylin Solution is formulated on the principle of the Gill’s Hematoxylin method and is available in three ready-to-use formulations:
Eosin Y is the most commonly used cytoplasmic stain. However, traditional alcoholic eosin solutions are highly flammable and harmful. The Hito Eosin-Y Aqueous Solution is a water-based, ready-to-use formulation that provides fast, safe, and reliable staining without the hazards of alcoholic solutions.
The Hito HE-Diff™ Solution is a water-based reagent used for hematoxylin staining differentiation.
In this method, traditional tap water is replaced by Hito Modified Scott’s Tap Water Substitute,
which enables bluing in a much shorter time and prevents tissue sections from detaching from slides.
Supplied as a 5x concentrated solution, it can be diluted to prepare up to 1250 ml of working solution.
Volume: 250 ml
