Bouin's Solution is a widely used fixative that enhances nuclear fixation. It is especially popular in embryonic studies and skin research due to its excellent preservation of nuclei and chromosomes. The solution is highly compatible with Verhoeff / Van Gieson stain thanks to its mordanting effect on tissue.
Bouin's Solution is particularly effective for fixation of testis, gastrointestinal tract, and endocrine tissue. It is also suitable for hematopoietic tissues and does not require dezenkerizing before staining, making it a versatile choice for histological applications.
Freshly prepared from paraformaldehyde powder and picric acid, Bouin's Solution retains its original quality for up to six months when stored at room temperature.
Bouin's solution is a fixative often used to enhance nuclear fixation. It is a popular fixative for embryonic studies and skin, due to its excellent preservation of nuclei and chromosomes. Bouin's is very compatible with the Verheoff / Van Gieson stain due to its mordanting effect on the tissue. Bouins Solution is particularly useful for fixation of tissues. It is often recommended for fixation of testis, gastrointestinal tract, and endocrine tissue. It can also be used to for fixation of hematopoietic tissues and does not require dezenkerizing before staining.