Hito Golgi-Cox OptimStain™ Kit Solution-3

This product is part of the Hito Golgi‑Cox OptimStain™ Kit.

Golgi‑Cox staining, or Golgi‑Cox impregnation, has long been recognized as one of the most elegant and effective procedures for studying the morphology of neurons and glia. It remains a primary technique for visualizing dendritic branching patterns and dendritic spines, allowing isolation and visualization of dendritic arbours from a random fraction of neurons in a given brain area.

Golgi techniques are not only valuable for pure anatomical studies, but are also widely used in research examining behavioral‑morphological relationships. In some studies, the Golgi‑Cox method has been applied to identify and study autonomic innervations in the heart, proving to be a useful tool for characterizing peripheral tissue morphology. However, the traditional Golgi‑Cox procedure is time‑consuming, requires a vibratome, yields low numbers of stained cells, and often produces unreliable results.

The Hito Golgi‑Cox OptimStain™ Kit offers a simple solution to these challenges. Based on the methods described by Glaser and Van der Loos, this kit dramatically improves the Golgi‑Cox technique. Procedures are simplified, processing time is greatly reduced, and users can employ a cryostat set at −19ºC. The kit delivers stable, high‑quality staining with minimal overstains and artifacts. A Standard Kit can be used for up to 50 mouse brains.

If a cryostat is not available, processed tissue can alternatively be prepared as paraffin sections using a microtome.

The Hito Golgi‑Cox OptimStain™ Kit has been extensively tested on brains, spinal cords, and hearts from several animal species, and has proven to be sensitive and reliable for demonstrating morphological details of neurons and glia. A simple and effective solution for your research.

Contents

Solution‑3	250 ml
Hito Golgi‑Cox OptimStain™ Kit Manual and MSDS