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The Perl’s stain is the most widely used method for detecting iron in biopsy specimens. It relies on the Prussian blue reaction. Historically introduced in 1867 by German pathologist Max Perls (1843–1881), the original formula used separate solutions of potassium ferrocyanide and acid to stain tissue. In this process, tissue is first treated with dilute hydrochloric acid to release ferric ions from binding proteins. The freed ions then react with potassium ferrocyanide to form insoluble Prussian blue dye (hydrated ferric ferrocyanide) in situ.
The Hito Perl’s Iron OptimStain™ Kit is designed based on the Perl’s stain method, offering a dramatic improvement over the traditional technique. Procedures are simplified, processing time is reduced, and the kit delivers stable, high-quality staining results. It provides a reliable and efficient solution for iron detection in histological research.
The Perl’s stain is the most widely used method for detecting iron in biopsy specimens, relying on the Prussian blue reaction. Historically introduced in 1867 by German pathologist Max Perls (1843–1881), the original formula used separate solutions of potassium ferrocyanide and acid to stain tissue. In this process, tissue is first treated with dilute hydrochloric acid to release ferric ions from binding proteins. The freed ions then react with potassium ferrocyanide to form insoluble Prussian blue dye (hydrated ferric ferrocyanide) in situ.
The Hito Perl’s Iron OptimStain™ Kit is designed based on the Perl’s stain method, offering a dramatic improvement over the traditional technique. Procedures are simplified, processing time is reduced, and the kit delivers stable, high-quality staining results. It provides a reliable and efficient solution for iron detection in histological research.
| Kit Contents | (for 150 slides) |
| Solution-1A | 125 ml |
| Solution-1B | 125 ml |
| Solution-2 | 125 ml |
| Solution-3 (Counterstain) | 30 ml |
| Dropper Bottle (30 ml Solution-3) | 1 |
| Staining Jars | 2 |
| User Manual and MSDS | 1 |
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Required Equipment / Materials (not included):
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